The account will be closed to coincide with the 85-year-old pope's formal departure from office at 8 p.m. local time, Vatican Radio said.
"It seems unimaginable that one could continue to use a communication tool so popular and powerful during the 'sede vacante' period," Vatican Radio said, referring to the time during which the seat will be empty.
"From choice, the Pontifex profile was not personalized, but it clearly refers to the person of the pope."
It's not yet clear whether whoever is chosen to succeed Benedict, who cited the frailty of age as the reason for his departure, will also use Twitter to reach out to some of the 1.5 billion Roman Catholics around the world.
On February 10, the day before the pope announced his shock resignation, @Pontifex tweeted: "We must trust in the mighty power of God's mercy. We are all sinners, but His grace transforms us and makes us new."
Only two tweets have been posted since in English, both relating to the Lent period.
According to Vatican Radio, Benedict is likely to offer his last tweet on February 27, the same day that he will give his final general audience to huge crowds expected to gather in St. Peter's Square in Vatican City.
If not then, perhaps one final thought will come from Benedict on February 28, "before retiring into silence," it suggests.
The pontiff is considering changing the Vatican constitution to allow a vote for his successor to begin before March 15, Vatican spokesman the Rev. Federico Lombardi said Wednesday.
Existing rules say the Roman Catholic Church's cardinals should start voting on a replacement from 15 to 20 days after the papal throne becomes vacant. With Benedict's resignation due to start on February 28, the cardinals' conclave ordinarily would start no sooner than March 15.
But Lombardi has said that because Benedict was leaving the papacy through resignation rather than death, the Vatican would explore the possibility of selecting a new pope sooner than normally prescribed.